Development of a desktop-sized thermal lens microscope
نویسندگان
چکیده
منابع مشابه
Confocal thermal-lens microscope.
The use of a confocal detection scheme in a dual-beam thermal-lens microscope is presented. The scheme allows the measurement of absorption factors down to 1.2 x 10(-7) in a 0.35-microm3 volume by use of a heating laser power of 100 mW incident upon the sample. Results are presented that prove that a 450-nm axial resolution is possible when a 1.2 water immersion objective lens with a N.A. of 1....
متن کاملDetermination of subyoctomole amounts of nonfluorescent molecules using a thermal lens microscope: subsingle-molecule determination.
The photothermal effect of an ultratrace amount of nonfluorescent molecules in liquid was determined by optimizing the optical arrangement for a thermal lens microscope. The optimized experimental setup could be determined from the evaluation of probing volume and the concentration of the sample solutions even when the expectation of the molecule number in the probing region was less than a sin...
متن کاملSuperlensing microscope objective lens.
Conventional microscope objective lenses are diffraction limited; they cannot resolve subdiffraction features of a size smaller than 250-300 nm under white lighting condition. New innovations are required to overcome this limitation. In this paper, we propose and demonstrate a new superlensing objective lens that possesses a resolution of 100 nm, which is a two-times resolution improvement over...
متن کاملDevelopment and Evaluation of Image Reconstruction Algorithms for a Novel Desktop SPECT System
Objective (s): Various iterative reconstruction algorithms in nuclear medicine have been introduced in the last three decades. For each new imaging system, it is wise to select appropriate image reconstruction algorithms and evaluate their performance. In this study, three approaches of image reconstruction were developed for a novel desktop open-gantry SPECT system, PERSPECT, to assess their p...
متن کاملChromatographic Separation of Nonfluorescent Molecules Using Extended-nano Channel and Differential Interference Contrast Thermal Lens Microscope
Chromatographic separation and detection of nonfluorescent molecules were integrated in a nanochannel. Our method, extended-nano chromatography realized high-performance separation using pressure-driven flow in a 100-nm scale nanochannel. A photothermal detector, differential interference contrast thermal lens microscope (DIC-TLM) was employed for detection of nonfluorescent samples. In particu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 2003
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.52.569